7WB0

PlmCasX-sgRNAv1-dsDNA ternary complex at nts loading state with flexible H2 domain

7WB0 の概要

• エントリーDOI: 10.2210/pdb7wb0/pdb
• EMDBエントリー: 32391
• 分子名称: TS-DNA, NTS-DNA, RNA (115-MER), ... (4 entities in total)
• 機能のキーワード: crispr, casx, sgrna, r-loop complex, rna binding protein, dna binding protein, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• 由来する生物種: Planctomycetes bacterium; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 176432.68

構造登録者

Zhang, S.,Liu, J.J.G. (登録日: 2021-12-15, 公開日: 2022-03-16, 最終更新日: 2025-07-02)

主引用文献

Tsuchida, C.A.,Zhang, S.,Doost, M.S.,Zhao, Y.,Wang, J.,O'Brien, E.,Fang, H.,Li, C.P.,Li, D.,Hai, Z.Y.,Chuck, J.,Brotzmann, J.,Vartoumian, A.,Burstein, D.,Chen, X.W.,Nogales, E.,Doudna, J.A.,Liu, J.G.
Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity.
Mol.Cell, 82:1199-1209.e6, 2022

PubMed Abstract: A compact protein with a size of <1,000 amino acids, the CRISPR-associated protein CasX is a fundamentally distinct RNA-guided nuclease when compared to Cas9 and Cas12a. Although it can induce RNA-guided genome editing in mammalian cells, the activity of CasX is less robust than that of the widely used S. pyogenes Cas9. Here, we show that structural features of two CasX homologs and their guide RNAs affect the R-loop complex assembly and DNA cleavage activity. Cryo-EM-based structural engineering of either the CasX protein or the guide RNA produced two new CasX genome editors (DpbCasX-R3-v2 and PlmCasX-R1-v2) with significantly improved DNA manipulation efficacy. These results advance both the mechanistic understanding of CasX and its application as a genome-editing tool.

PubMed: 35219382
DOI: 10.1016/j.molcel.2022.02.002

実験手法

ELECTRON MICROSCOPY (3.2 Å)