7WAZ

PlmCasX-sgRNAv1-dsDNA ternary complex at ts loading state

Summary for 7WAZ

Entry DOI	10.2210/pdb7waz/pdb
EMDB information	32390
Descriptor	dPlmCasX, NTS-DNA, RNA (115-MER), ... (4 entities in total)
Functional Keywords	crispr, casx, sgrna, r-loop complex, rna binding protein, rna binding protein-dna-rna complex, dna binding protein, rna binding protein-dna-rna
Biological source	Planctomycetes bacterium More
Total number of polymer chains	4
Total formula weight	176432.68
Authors	Zhang, S.,Liu, J.J.G. (deposition date: 2021-12-15, release date: 2022-03-16, Last modification date: 2024-06-26)
Primary citation	Tsuchida, C.A.,Zhang, S.,Doost, M.S.,Zhao, Y.,Wang, J.,O'Brien, E.,Fang, H.,Li, C.P.,Li, D.,Hai, Z.Y.,Chuck, J.,Brotzmann, J.,Vartoumian, A.,Burstein, D.,Chen, X.W.,Nogales, E.,Doudna, J.A.,Liu, J.G. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Mol.Cell, 82:1199-1209.e6, 2022 Cited by PubMed: 35219382 DOI: 10.1016/j.molcel.2022.02.002 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.4 Å)

Structure validation