7WAG

Crystal structure of MurJ squeezed form

7WAG の概要

• エントリーDOI: 10.2210/pdb7wag/pdb
• 分子名称: Lipid II flippase MurJ, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: 14 transmembrane helices, inner membrane, lipid ii, mop superfamily, lipid transport
• 由来する生物種: Escherichia coli K-12
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 61320.55

構造登録者

Tsukazaki, T.,Kohga, H.,Tanaka, Y.,Yoshikaie, K.,Taniguchi, K.,Fujimoto, K. (登録日: 2021-12-14, 公開日: 2022-06-01, 最終更新日: 2023-11-29)

主引用文献

Kohga, H.,Mori, T.,Tanaka, Y.,Yoshikaie, K.,Taniguchi, K.,Fujimoto, K.,Fritz, L.,Schneider, T.,Tsukazaki, T.
Crystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms.
Structure, 30:1088-1097.e3, 2022

PubMed Abstract: The bacterial peptidoglycan enclosing the cytoplasmic membrane is a fundamental cellular architecture. The integral membrane protein MurJ plays an essential role in flipping the cell wall building block Lipid II across the cytoplasmic membrane for peptidoglycan biosynthesis. Previously reported crystal structures of MurJ have elucidated its V-shaped inward- or outward-facing forms with an internal cavity for substrate binding. MurJ transports Lipid II using its cavity through conformational transitions between these two forms. Here, we report two crystal structures of inward-facing forms from Arsenophonus endosymbiont MurJ and an unprecedented crystal structure of Escherichia coli MurJ in a "squeezed" form, which lacks a cavity to accommodate the substrate, mainly because of the increased proximity of transmembrane helices 2 and 8. Subsequent molecular dynamics simulations supported the hypothesis that the squeezed form is an intermediate conformation. This study fills a gap in our understanding of the Lipid II flipping mechanism.

PubMed: 35660157
DOI: 10.1016/j.str.2022.05.008

実験手法

X-RAY DIFFRACTION (2.55 Å)