7W5C

Crystal structure of Mitogen Activated Protein Kinase 4 (MPK4) from Arabidopsis thaliana

7W5C の概要

• エントリーDOI: 10.2210/pdb7w5c/pdb
• 分子名称: Mitogen-activated protein kinase 4, Mitogen-activated protein kinase kinase 1, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (5 entities in total)
• 機能のキーワード: mpk4, kinase, atpase, plant protein, transferase
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42862.80

構造登録者

Arold, S.T.,Hameed, U.F.S. (登録日: 2021-11-30, 公開日: 2022-12-07, 最終更新日: 2023-11-29)

主引用文献

Siodmak, A.,Shahul Hameed, U.F.,Rayapuram, N.,Volz, R.,Boudsocq, M.,Alharbi, S.,Alhoraibi, H.,Lee, Y.H.,Blilou, I.,Arold, S.T.,Hirt, H.
Essential role of the CD docking motif of MPK4 in plant immunity, growth, and development.
New Phytol., 239:1112-1126, 2023

PubMed Abstract: MAPKs are universal eukaryotic signaling factors whose functioning is assumed to depend on the recognition of a common docking motif (CD) by its activators, substrates, and inactivators. We studied the role of the CD domain of Arabidopsis MPK4 by performing interaction studies and determining the ligand-bound MPK4 crystal structure. We revealed that the CD domain of MPK4 is essential for interaction and activation by its upstream MAPKKs MKK1, MKK2, and MKK6. Cys181 in the CD site of MPK4 was shown to become sulfenylated in response to reactive oxygen species in vitro. To test the function of C181 in vivo, we generated wild-type (WT) MPK4-C181, nonsulfenylatable MPK4-C181S, and potentially sulfenylation mimicking MPK4-C181D lines in the mpk4 knockout background. We analyzed the phenotypes in growth, development, and stress responses, revealing that MPK4-C181S has WT activity and complements the mpk4 phenotype. By contrast, MPK4-C181D cannot be activated by upstream MAPKK and cannot complement the phenotypes of mpk4. Our findings show that the CD motif is essential and is required for activation by upstream MAPKK for MPK4 function. Furthermore, growth, development, or immunity functions require upstream activation of the MPK4 protein kinase.

PubMed: 37243525
DOI: 10.1111/nph.18989

実験手法

X-RAY DIFFRACTION (2.201 Å)