7VSO

Serial Femtosecond Crystallography (SFX) of Ground State Bacteriorhodopsin Crystallized from Bicelles in Complex with HAD16 Determined Using 7-keV X-ray Free Electron Laser (XFEL) at SACLA

Summary for 7VSO

• Entry DOI: 10.2210/pdb7vso/pdb
• Descriptor: Bacteriorhodopsin, DECANE, nonane, ... (12 entities in total)
• Functional Keywords: proton pump, transport protein, retinal, proton transport, membrane protein
• Biological source: Halobacterium salinarum
• Total number of polymer chains: 1
• Total formula weight: 31065.81

Authors

Mizohata, E.,Nakane, T.,Hanashima, S. (deposition date: 2021-10-27, release date: 2022-02-16, Last modification date: 2023-11-29)

Primary citation

Hanashima, S.,Nakane, T.,Mizohata, E.
Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.
Membranes (Basel), 11:-, 2021

PubMed Abstract: Membrane proteins reside in the lipid bilayer of biomembranes and the structure and function of these proteins are closely related to their interactions with lipid molecules. Structural analyses of interactions between membrane proteins and lipids or detergents that constitute biological or artificial model membranes are important for understanding the functions and physicochemical properties of membrane proteins and biomembranes. Determination of membrane protein structures is much more difficult when compared with that of soluble proteins, but the development of various new technologies has accelerated the elucidation of the structure-function relationship of membrane proteins. This review summarizes the development of heavy atom derivative detergents and lipids that can be used for structural analysis of membrane proteins and their interactions with detergents/lipids, including their application with X-ray free-electron laser crystallography.

PubMed: 34832053
DOI: 10.3390/membranes11110823

Experimental method

X-RAY DIFFRACTION (2.35 Å)