7VR1

Cryo-EM structure of the ATP-binding cassette sub-family D member 1 from Homo sapiens

Summary for 7VR1

• Entry DOI: 10.2210/pdb7vr1/pdb
• EMDB information: 32096
• Descriptor: ATP-binding cassette sub-family D member 1 (1 entity in total)
• Functional Keywords: membrane protein, lipid transport, translocase
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 166081.73

Authors

Yang, G.H.,Jia, Y.T.,Zhang, Y.M. (deposition date: 2021-10-21, release date: 2022-10-26, Last modification date: 2025-06-25)

Primary citation

Jia, Y.,Zhang, Y.,Wang, W.,Lei, J.,Ying, Z.,Yang, G.
Structural and functional insights of the human peroxisomal ABC transporter ALDP.
Elife, 11:-, 2022

PubMed Abstract: Adrenoleukodystrophy protein (ALDP) is responsible for the transport of very-long-chain fatty acids (VLCFAs) and corresponding CoA-esters across the peroxisomal membrane. Dysfunction of ALDP leads to peroxisomal metabolic disorder exemplified by X-linked adrenoleukodystrophy (ALD). Hundreds of ALD-causing mutations have been identified on ALDP. However, the pathogenic mechanisms of these mutations are restricted to clinical description due to limited structural and biochemical characterization. Here we report the cryo-electron microscopy structure of human ALDP with nominal resolution at 3.4 Å. ALDP exhibits a cytosolic-facing conformation. Compared to other lipid ATP-binding cassette transporters, ALDP has two substrate binding cavities formed by the transmembrane domains. Such structural organization may be suitable for the coordination of VLCFAs. Based on the structure, we performed integrative analysis of the cellular trafficking, protein thermostability, ATP hydrolysis, and the transport activity of representative mutations. These results provide a framework for understanding the working mechanism of ALDP and pathogenic roles of disease-associated mutations.

PubMed: 36374178
DOI: 10.7554/eLife.75039

Experimental method

ELECTRON MICROSCOPY (3.4 Å)