7VI8
Crystal structure of ChbG
Summary for 7VI8
| Entry DOI | 10.2210/pdb7vi8/pdb |
| Descriptor | Chitooligosaccharide deacetylase, ZINC ION, ACETATE ION, ... (4 entities in total) |
| Functional Keywords | chbg, chitin catabolism, chitin deacetylase, chitobiose operon, klebsiella pneumoniae, hydrolase |
| Biological source | Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) |
| Total number of polymer chains | 2 |
| Total formula weight | 56109.02 |
| Authors | Lee, S.Y.,Park, H.H. (deposition date: 2021-09-26, release date: 2022-09-07, Last modification date: 2023-11-29) |
| Primary citation | Lee, S.Y.,Pardhe, B.D.,Oh, T.J.,Park, H.H. Crystal structure of ChbG from Klebsiella pneumoniae reveals the molecular basis of diacetylchitobiose deacetylation. Commun Biol, 5:862-862, 2022 Cited by PubMed Abstract: The chitobiose (chb) operon is involved in the synthesis of chitooligosaccharide and is comprised of a BCARFG gene cluster. ChbG encodes a chitooligosaccharide deacetylase (CDA) which catalyzes the removal of one acetyl group from N,N'-diacetylchitobiose. It is considered a novel type of CDA due to its lack of sequence homology. Although there are various structural studies of CDAs linked to the kinetic properties of the enzyme, the structural information of ChbG is unavailable. In this study, the crystal structure of ChbG from Klebsiella pneumoniae is provided. The molecular basis of deacetylation of diacetylchitobiose by ChbG is determined based on structural analysis, mutagenesis, biophysical analysis, and in silico docking of the substrate, diacetylchitobiose. This study contributes towards a deeper understanding of chitin and chitosan biology, as well as provides a platform to engineer CDA biocatalysts. PubMed: 36002585DOI: 10.1038/s42003-022-03824-9 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.83 Å) |
Structure validation
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