7VF6
The crystal structure of PurZ0
Summary for 7VF6
| Entry DOI | 10.2210/pdb7vf6/pdb |
| Descriptor | PurA-like adenylosuccinate synthetase, PHOSPHATE ION, GUANOSINE-5'-DIPHOSPHATE, ... (6 entities in total) |
| Functional Keywords | pura-like adenylosuccinate synthetase, ligase |
| Biological source | Gordonia phage Archimedes |
| Total number of polymer chains | 2 |
| Total formula weight | 82528.99 |
| Authors | |
| Primary citation | Tong, Y.,Wu, X.,Liu, Y.,Chen, H.,Zhou, Y.,Jiang, L.,Li, M.,Zhao, S.,Zhang, Y. Alternative Z-genome biosynthesis pathway shows evolutionary progression from Archaea to phage. Nat Microbiol, 8:1330-1338, 2023 Cited by PubMed Abstract: Many bacteriophages evade bacterial immune recognition by substituting adenine with 2,6-diaminopurine (Z) in their genomes. The Z-genome biosynthetic pathway involves PurZ that belongs to the PurA (adenylosuccinate synthetase) family and bears particular similarity to archaeal PurA. However, how the transition of PurA to PurZ occurred during evolution is not clear; recapturing this process may shed light on the origin of Z-containing phages. Here we describe the computer-guided identification and biochemical characterization of a naturally existing PurZ variant, PurZ0, which uses guanosine triphosphate as the phosphate donor rather than the ATP used by PurZ. The atomic resolution structure of PurZ0 reveals a guanine nucleotide binding pocket highly analogous to that of archaeal PurA. Phylogenetic analyses suggest PurZ0 as an intermediate during the evolution of archaeal PurA to phage PurZ. Maintaining the balance of different purines necessitates further evolvement of guanosine triphosphate-using PurZ0 to ATP-using PurZ in adaptation to Z-genome life. PubMed: 37308591DOI: 10.1038/s41564-023-01410-1 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.703 Å) |
Structure validation
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