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7VC1

Toxoplasma gondii Prolyl-tRNA Synthetase (TgPRS) in Complex with inhibitor L95 and L-proline in space group P21

Summary for 7VC1
Entry DOI10.2210/pdb7vc1/pdb
DescriptorProlyl-tRNA synthetase (ProRS), ~{N}-[4-[(3~{S})-3-cyano-3-cyclopropyl-2-oxidanylidene-pyrrolidin-1-yl]-6-methyl-pyridin-2-yl]-2-phenyl-ethanamide, PROLINE, ... (7 entities in total)
Functional Keywordsprotein translation, inhibitor, prs, atp pocket, ligase, ligase-ligase inhibitor complex, ligase/ligase inhibitor
Biological sourceToxoplasma gondii
Total number of polymer chains2
Total formula weight117310.76
Authors
Yogavel, M.,Malhotra, N.,Sharma, A. (deposition date: 2021-09-01, release date: 2022-09-07, Last modification date: 2023-11-29)
Primary citationYogavel, M.,Bougdour, A.,Mishra, S.,Malhotra, N.,Chhibber-Goel, J.,Bellini, V.,Harlos, K.,Laleu, B.,Hakimi, M.A.,Sharma, A.
Targeting prolyl-tRNA synthetase via a series of ATP-mimetics to accelerate drug discovery against toxoplasmosis.
Plos Pathog., 19:e1011124-e1011124, 2023
Cited by
PubMed Abstract: The prolyl-tRNA synthetase (PRS) is a validated drug target for febrifugine and its synthetic analog halofuginone (HFG) against multiple apicomplexan parasites including Plasmodium falciparum and Toxoplasma gondii. Here, a novel ATP-mimetic centered on 1-(pyridin-4-yl) pyrrolidin-2-one (PPL) scaffold has been validated to bind to Toxoplasma gondii PRS and kill toxoplasma parasites. PPL series exhibited potent inhibition at the cellular (T. gondii parasites) and enzymatic (TgPRS) levels compared to the human counterparts. Cell-based chemical mutagenesis was employed to determine the mechanism of action via a forward genetic screen. Tg-resistant parasites were analyzed with wild-type strain by RNA-seq to identify mutations in the coding sequence conferring drug resistance by computational analysis of variants. DNA sequencing established two mutations, T477A and T592S, proximal to terminals of the PPL scaffold and not directly in the ATP, tRNA, or L-pro sites, as supported by the structural data from high-resolution crystal structures of drug-bound enzyme complexes. These data provide an avenue for structure-based activity enhancement of this chemical series as anti-infectives.
PubMed: 36854028
DOI: 10.1371/journal.ppat.1011124
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.892 Å)
Structure validation

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