7V9E
Crystal structure of a methyl transferase ribozyme
Summary for 7V9E
| Entry DOI | 10.2210/pdb7v9e/pdb |
| Descriptor | RNA (68-MER), GUANINE, BARIUM ION, ... (5 entities in total) |
| Functional Keywords | methyl transferase, ribozyme, riboswitch, rna |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 23317.18 |
| Authors | Deng, J.,Lilley, D.M.J.,Huang, L. (deposition date: 2021-08-25, release date: 2022-03-23, Last modification date: 2024-05-29) |
| Primary citation | Deng, J.,Wilson, T.J.,Wang, J.,Peng, X.,Li, M.,Lin, X.,Liao, W.,Lilley, D.M.J.,Huang, L. Structure and mechanism of a methyltransferase ribozyme. Nat.Chem.Biol., 18:556-564, 2022 Cited by PubMed Abstract: Known ribozymes in contemporary biology perform a limited range of chemical catalysis, but in vitro selection has generated species that catalyze a broader range of chemistry; yet, there have been few structural and mechanistic studies of selected ribozymes. A ribozyme has recently been selected that can catalyze a site-specific methyl transfer reaction. We have solved the crystal structure of this ribozyme at a resolution of 2.3 Å, showing how the RNA folds to generate a very specific binding site for the methyl donor substrate. The structure immediately suggests a catalytic mechanism involving a combination of proximity and orientation and nucleobase-mediated general acid catalysis. The mechanism is supported by the pH dependence of the rate of catalysis. A selected methyltransferase ribozyme can thus use a relatively sophisticated catalytic mechanism, broadening the range of known RNA-catalyzed chemistry. PubMed: 35301479DOI: 10.1038/s41589-022-00982-z PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
Download full validation report
