7UZT

Crystal structure of the human astrovirus MLB1 capsid protein spike domain at 1.86-A resolution

Summary for 7UZT

• Entry DOI: 10.2210/pdb7uzt/pdb
• Descriptor: Capsid polyprotein VP90 (2 entities in total)
• Functional Keywords: capsid protein, spike domain, projection domain, viral protein
• Biological source: Astrovirus MLB1
• Total number of polymer chains: 3
• Total formula weight: 88924.57

Authors

Delgado-Cunningham, K.,DuBois, R. (deposition date: 2022-05-09, release date: 2022-11-09, Last modification date: 2024-05-22)

Primary citation

Delgado-Cunningham, K.,Lopez, T.,Khatib, F.,Arias, C.F.,DuBois, R.M.
Structure of the divergent human astrovirus MLB capsid spike.
Structure, 30:1573-1581.e3, 2022

PubMed Abstract: Despite their worldwide prevalence and association with human disease, the molecular bases of human astrovirus (HAstV) infection and evolution remain poorly characterized. Here, we report the structure of the capsid protein spike of the divergent HAstV MLB clade (HAstV MLB). While the structure shares a similar folding topology with that of classical-clade HAstV spikes, it is otherwise strikingly different. We find no evidence of a conserved receptor-binding site between the MLB and classical HAstV spikes, suggesting that MLB and classical HAstVs utilize different receptors for host-cell attachment. We provide evidence for this hypothesis using a novel HAstV infection competition assay. Comparisons of the HAstV MLB spike structure with structures predicted from its sequence reveal poor matches, but template-based predictions were surprisingly accurate relative to machine-learning-based predictions. Our data provide a foundation for understanding the mechanisms of infection by diverse HAstVs and can support structure determination in similarly unstudied systems.

PubMed: 36417907
DOI: 10.1016/j.str.2022.10.010

Experimental method

X-RAY DIFFRACTION (1.86 Å)