7UXB

Human triosephosphate isomerase mutant G122R

Summary for 7UXB

Entry DOI	10.2210/pdb7uxb/pdb
Descriptor	Triosephosphate isomerase, ISOPROPYL ALCOHOL, GLYCEROL, ... (4 entities in total)
Functional Keywords	enzyme, glycolysis, isomerase
Biological source	Homo sapiens (human)
Total number of polymer chains	2
Total formula weight	54031.68
Authors	Romero, J.M. (deposition date: 2022-05-05, release date: 2023-05-24, Last modification date: 2024-11-20)
Primary citation	Romero, J.M. Structural analysis of the TPI-Manchester, a thermolabile variant of human triosephosphate isomerase. Arch.Biochem.Biophys., 761:110156-110156, 2024 Cited by PubMed Abstract: Human triosephosphate isomerase G122R, also known as TPI-Manchester, is a thermolabile variant detected in a screening of more than 3400 individuals from a population in Ann Arbor, Michigan. Here, the crystallographic structure of G122R was solved to determine the molecular basis of its thermal stability. Structural analysis revealed an increase in the flexibility of residues at the dimer interface, even though R122 is about 20 Å away, suggesting that long-range electrostatic interactions may play a key role in the mutation effect. PubMed: 39299479 DOI: 10.1016/j.abb.2024.110156 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2 Å)

Structure validation