7UOW

SARS-Cov2 S protein structure in complex with neutralizing monoclonal antibody 034_32

7UOW の概要

• エントリーDOI: 10.2210/pdb7uow/pdb
• EMDBエントリー: 26656
• 分子名称: Spike glycoprotein, Monoclonal antibody 034_32 heavy chain, Monoclonal antibody 034_32 light chain, ... (5 entities in total)
• 機能のキーワード: sars-cov2 6p spike protein, immune complex, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 555716.19

構造登録者

Patel, A.,Ortlund, E. (登録日: 2022-04-14, 公開日: 2023-04-19, 最終更新日: 2024-11-13)

主引用文献

Patel, A.,Kumar, S.,Lai, L.,Chakravarthy, C.,Valanparambil, R.,Reddy, E.S.,Gottimukkala, K.,Bajpai, P.,Raju, D.R.,Edara, V.V.,Davis-Gardner, M.E.,Linderman, S.,Dixit, K.,Sharma, P.,Mantus, G.,Cheedarla, N.,Verkerke, H.P.,Frank, F.,Neish, A.S.,Roback, J.D.,Davis, C.W.,Wrammert, J.,Ahmed, R.,Suthar, M.S.,Sharma, A.,Murali-Krishna, K.,Chandele, A.,Ortlund, E.A.
Molecular basis of SARS-CoV-2 Omicron variant evasion from shared neutralizing antibody response.
Structure, 31:801-811.e5, 2023

PubMed Abstract: Understanding the molecular features of neutralizing epitopes is important for developing vaccines/therapeutics against emerging SARS-CoV-2 variants. We describe three monoclonal antibodies (mAbs) generated from COVID-19 recovered individuals during the first wave of the pandemic in India. These mAbs had publicly shared near germline gene usage and potently neutralized Alpha and Delta, poorly neutralized Beta, and failed to neutralize Omicron BA.1 SARS-CoV-2 variants. Structural analysis of these mAbs in complex with trimeric spike protein showed that all three mAbs bivalently bind spike with two mAbs targeting class 1 and one targeting a class 4 receptor binding domain epitope. The immunogenetic makeup, structure, and function of these mAbs revealed specific molecular interactions associated with the potent multi-variant binding/neutralization efficacy. This knowledge shows how mutational combinations can affect the binding or neutralization of an antibody, which in turn relates to the efficacy of immune responses to emerging SARS-CoV-2 escape variants.

PubMed: 37167972
DOI: 10.1016/j.str.2023.04.010

実験手法

ELECTRON MICROSCOPY (4.4 Å)