7UNC

Pol II-DSIF-SPT6-PAF1c-TFIIS complex with rewrapped nucleosome

これはPDB形式変換不可エントリーです。

7UNC の概要

• エントリーDOI: 10.2210/pdb7unc/pdb
• EMDBエントリー: 26620
• 分子名称: DNA-directed RNA polymerase subunit, RPB10, RPB11, ... (30 entities in total)
• 機能のキーワード: chromatin, nucleosome, transcription, intermediate
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 1577916.38

構造登録者

Filipovski, M.,Vos, S.M.,Farnung, L. (登録日: 2022-04-10, 公開日: 2022-10-19, 最終更新日: 2024-10-23)

主引用文献

Filipovski, M.,Soffers, J.H.M.,Vos, S.M.,Farnung, L.
Structural basis of nucleosome retention during transcription elongation.
Science, 376:1313-1316, 2022

PubMed Abstract: In eukaryotes, RNA polymerase (Pol) II transcribes chromatin and must move past nucleosomes, often resulting in nucleosome displacement. How Pol II unwraps the DNA from nucleosomes to allow transcription and how DNA rewraps to retain nucleosomes has been unclear. Here, we report the 3.0-angstrom cryo-electron microscopy structure of a mammalian Pol II-DSIF-SPT6-PAF1c-TFIIS-nucleosome complex stalled 54 base pairs within the nucleosome. The structure provides a mechanistic basis for nucleosome retention during transcription elongation where upstream DNA emerging from the Pol II cleft has rewrapped the proximal side of the nucleosome. The structure uncovers a direct role for Pol II and transcription elongation factors in nucleosome retention and explains how nucleosomes are retained to prevent the disruption of chromatin structure across actively transcribed genes.

PubMed: 35709268
DOI: 10.1126/science.abo3851

実験手法

ELECTRON MICROSCOPY (3 Å)