7UG6

Cryo-EM structure of pre-60S ribosomal subunit, unmethylated G2922

これはPDB形式変換不可エントリーです。

7UG6 の概要

• エントリーDOI: 10.2210/pdb7ug6/pdb
• EMDBエントリー: 26485
• 分子名称: 25S rRNA, 60S ribosomal protein L7-A, 60S ribosomal protein L8-A, ... (54 entities in total)
• 機能のキーワード: ribosome, ribosome biogenesis, rrna modification, methylation
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 52
• 化学式量合計: 2338390.00

構造登録者

Yelland, J.N.,Bravo, J.P.K.,Black, J.J.B.,Taylor, D.W.,Johnson, A.W. (登録日: 2022-03-24, 公開日: 2022-12-21, 最終更新日: 2024-06-12)

主引用文献

Yelland, J.N.,Bravo, J.P.K.,Black, J.J.,Taylor, D.W.,Johnson, A.W.
A single 2'-O-methylation of ribosomal RNA gates assembly of a functional ribosome.
Nat.Struct.Mol.Biol., 30:91-98, 2023

PubMed Abstract: RNA modifications are widespread in biology and abundant in ribosomal RNA. However, the importance of these modifications is not well understood. We show that methylation of a single nucleotide, in the catalytic center of the large subunit, gates ribosome assembly. Massively parallel mutational scanning of the essential nuclear GTPase Nog2 identified important interactions with rRNA, particularly with the 2'-O-methylated A-site base Gm2922. We found that methylation of G2922 is needed for assembly and efficient nuclear export of the large subunit. Critically, we identified single amino acid changes in Nog2 that completely bypass dependence on G2922 methylation and used cryoelectron microscopy to directly visualize how methylation flips Gm2922 into the active site channel of Nog2. This work demonstrates that a single RNA modification is a critical checkpoint in ribosome biogenesis, suggesting that such modifications can play an important role in regulation and assembly of macromolecular machines.

PubMed: 36536102
DOI: 10.1038/s41594-022-00891-8

実験手法

ELECTRON MICROSCOPY (2.9 Å)