7UBA

Structure of fungal Hop1 CBR domain

7UBA の概要

• エントリーDOI: 10.2210/pdb7uba/pdb
• 分子名称: HORMA domain-containing protein, ZINC ION, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (6 entities in total)
• 機能のキーワード: meiosis, hormad, phd, winged helix, dna binding protein
• 由来する生物種: Vanderwaltozyma polyspora DSM 70294 (Kluyveromyces polysporus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24997.16

構造登録者

Ur, S.N.,Corbett, K.D. (登録日: 2022-03-14, 公開日: 2023-03-29, 最終更新日: 2024-04-10)

主引用文献

Milano, C.R.,Ur, S.N.,Gu, Y.,Zhang, J.,Allison, R.,Brown, G.,Neale, M.J.,Tromer, E.C.,Corbett, K.D.,Hochwagen, A.
Chromatin binding by HORMAD proteins regulates meiotic recombination initiation.
Embo J., 43:836-867, 2024

PubMed Abstract: The meiotic chromosome axis coordinates chromosome organization and interhomolog recombination in meiotic prophase and is essential for fertility. In S. cerevisiae, the HORMAD protein Hop1 mediates the enrichment of axis proteins at nucleosome-rich islands through a central chromatin-binding region (CBR). Here, we use cryoelectron microscopy to show that the Hop1 CBR directly recognizes bent nucleosomal DNA through a composite interface in its PHD and winged helix-turn-helix domains. Targeted disruption of the Hop1 CBR-nucleosome interface causes a localized reduction of axis protein binding and meiotic DNA double-strand breaks (DSBs) in axis islands and leads to defects in chromosome synapsis. Synthetic effects with mutants of the Hop1 regulator Pch2 suggest that nucleosome binding delays a conformational switch in Hop1 from a DSB-promoting, Pch2-inaccessible state to a DSB-inactive, Pch2-accessible state to regulate the extent of meiotic DSB formation. Phylogenetic analyses of meiotic HORMADs reveal an ancient origin of the CBR, suggesting that the mechanisms we uncover are broadly conserved.

PubMed: 38332377
DOI: 10.1038/s44318-024-00034-3

実験手法

X-RAY DIFFRACTION (1.599 Å)