7UBA
Structure of fungal Hop1 CBR domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.283 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.389, 38.917, 68.981 |
Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
Resolution | 32.600 - 1.599 |
R-factor | 0.1451 |
Rwork | 0.144 |
R-free | 0.16350 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.309 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.600 | 1.610 |
High resolution limit [Å] | 1.510 | 1.510 |
Rmeas | 0.057 | 0.804 |
Number of reflections | 65701 | 7028 |
<I/σ(I)> | 13.93 | |
Completeness [%] | 98.5 | 61 |
Redundancy | 3.34 | 1.97 |
CC(1/2) | 0.999 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH 6.5, 30% PEG 400 |