7U5N

Cryo-EM Structure of Glutamine Synthetase

Summary for 7U5N

• Entry DOI: 10.2210/pdb7u5n/pdb
• EMDB information: 26356
• Descriptor: Glutamine synthetase, MANGANESE (II) ION (2 entities in total)
• Functional Keywords: gs, glutamine synthetase, glul, peptide binding protein
• Biological source: Bos taurus (cattle)
• Total number of polymer chains: 10
• Total formula weight: 421403.52

Authors

Morgan, C.E.,Yu, E.W.,Zhang, Z. (deposition date: 2022-03-02, release date: 2022-12-14, Last modification date: 2024-06-12)

Primary citation

Morgan, C.E.,Zhang, Z.,Miyagi, M.,Golczak, M.,Yu, E.W.
Toward structural-omics of the bovine retinal pigment epithelium.
Cell Rep, 41:111876-111876, 2022

PubMed Abstract: The use of an integrated systems biology approach to investigate tissues and organs has been thought to be impracticable in the field of structural biology, where the techniques mainly focus on determining the structure of a particular biomacromolecule of interest. Here, we report the use of cryoelectron microscopy (cryo-EM) to define the composition of a raw bovine retinal pigment epithelium (RPE) lysate. From this sample, we simultaneously identify and solve cryo-EM structures of seven different RPE enzymes whose functions affect neurotransmitter recycling, iron metabolism, gluconeogenesis, glycolysis, axonal development, and energy homeostasis. Interestingly, dysfunction of these important proteins has been directly linked to several neurodegenerative disorders, including Huntington's disease, amyotrophic lateral sclerosis (ALS), Parkinson's disease, Alzheimer's disease, and schizophrenia. Our work underscores the importance of cryo-EM in facilitating tissue and organ proteomics at the atomic level.

PubMed: 36577381
DOI: 10.1016/j.celrep.2022.111876

Experimental method

ELECTRON MICROSCOPY (2.58 Å)