7U0F

HIV-1 Rev in complex with tubulin

7U0F の概要

• エントリーDOI: 10.2210/pdb7u0f/pdb
• EMDBエントリー: 26257
• 分子名称: Tubulin alpha-1A chain, Tubulin beta chain, Protein Rev (3 entities in total)
• 機能のキーワード: rev, tubulin, hiv-1, viral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 278392.19

構造登録者

Eren, E. (登録日: 2022-02-18, 公開日: 2023-08-23, 最終更新日: 2023-10-18)

主引用文献

Eren, E.,Watts, N.R.,Randazzo, D.,Palmer, I.,Sackett, D.L.,Wingfield, P.T.
Structural basis of microtubule depolymerization by the kinesin-like activity of HIV-1 Rev.
Structure, 31:1233-, 2023

PubMed Abstract: HIV-1 Rev is an essential regulatory protein that transports unspliced and partially spliced viral mRNAs from the nucleus to the cytoplasm for the expression of viral structural proteins. During its nucleocytoplasmic shuttling, Rev interacts with several host proteins to use the cellular machinery for the advantage of the virus. Here, we report the 3.5 Å cryo-EM structure of a 4.8 MDa Rev-tubulin ring complex. Our structure shows that Rev's arginine-rich motif (ARM) binds to both the acidic surfaces and the C-terminal tails of α/β-tubulin. The Rev-tubulin interaction is functionally homologous to that of kinesin-13, potently destabilizing microtubules at sub-stoichiometric levels. Expression of Rev in astrocytes and HeLa cells shows that it can modulate the microtubule cytoskeleton within the cellular environment. These results show a previously undefined regulatory role of Rev.

PubMed: 37572662
DOI: 10.1016/j.str.2023.07.009

実験手法

ELECTRON MICROSCOPY (3.53 Å)