7U06

Structure of the yeast TRAPPII-Rab11/Ypt32 complex in the closed/open state (composite structure)

これはPDB形式変換不可エントリーです。

7U06 の概要

• エントリーDOI: 10.2210/pdb7u06/pdb
• EMDBエントリー: 26233 26234 26235 26236 26237 26238 26239 26240 26241 26242 26243 26244 26245 26246 26247 26248 26249 26250 26251 26252 26253 26255
• 分子名称: Trafficking protein particle complex II-specific subunit 65, Trafficking protein particle complex II-specific subunit 130, Trafficking protein particle complex II-specific subunit 120, ... (14 entities in total)
• 機能のキーワード: complex, gtpase, guanosine exchange factor, gef, protein transport
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 27
• 化学式量合計: 1165950.19

構造登録者

Bagde, S.R.,Fromme, J.C. (登録日: 2022-02-17, 公開日: 2022-04-27, 最終更新日: 2024-11-13)

主引用文献

Bagde, S.R.,Fromme, J.C.
Structure of a TRAPPII-Rab11 activation intermediate reveals GTPase substrate selection mechanisms.
Sci Adv, 8:eabn7446-eabn7446, 2022

PubMed Abstract: Rab1 and Rab11 are essential regulators of the eukaryotic secretory and endocytic recycling pathways. The transport protein particle (TRAPP) complexes activate these guanosine triphosphatases via nucleotide exchange using a shared set of core subunits. The basal specificity of the TRAPP core is toward Rab1, yet the TRAPPII complex is specific for Rab11. A steric gating mechanism has been proposed to explain TRAPPII counterselection against Rab1. Here, we present cryo-electron microscopy structures of the 22-subunit TRAPPII complex from budding yeast, including a TRAPPII-Rab11 nucleotide exchange intermediate. The Trs130 subunit provides a "leg" that positions the active site distal to the membrane surface, and this leg is required for steric gating. The related TRAPPIII complex is unable to activate Rab11 because of a repulsive interaction, which TRAPPII surmounts using the Trs120 subunit as a "lid" to enclose the active site. TRAPPII also adopts an open conformation enabling Rab11 to access and exit from the active site chamber.

PubMed: 35559680
DOI: 10.1126/sciadv.abn7446

実験手法

ELECTRON MICROSCOPY (4.2 Å)