7TVW

Crystal structure of Arabidopsis thaliana DLK2

Summary for 7TVW

• Entry DOI: 10.2210/pdb7tvw/pdb
• Descriptor: Alpha/beta-Hydrolases superfamily protein (2 entities in total)
• Functional Keywords: sesquiterpene lactone perception, hydrolase
• Biological source: Arabidopsis thaliana (thale cress)
• Total number of polymer chains: 1
• Total formula weight: 30207.77

Authors

Burger, M.,Chory, J. (deposition date: 2022-02-06, release date: 2022-09-28, Last modification date: 2023-10-18)

Primary citation

Burger, M.,Honda, K.,Kondoh, Y.,Hong, S.,Watanabe, N.,Osada, H.,Chory, J.
Crystal structure of Arabidopsis DWARF14-LIKE2 (DLK2) reveals a distinct substrate binding pocket architecture.
Plant Direct, 6:e446-e446, 2022

PubMed Abstract: In , the Sigma factor B regulator RsbQ-like family of α/β hydrolases contains the strigolactone (SL) receptor DWARF14 (AtD14), the karrikin receptor KARRIKIN INSENSITIVE2 (AtKAI2), and DWARF14-LIKE2 (AtDLK2), a protein of unknown function. Despite very similar protein folds, AtD14 and AtKAI2 differ in size and architecture of their ligand binding pockets, influencing their substrate specificity. We present the 1.5 Å crystal structure of AtDLK2, revealing the smallest ligand binding pocket in the protein family, bordered by two unique glycine residues. We identified a gatekeeper residue in the protein's lid domain and present a pyrrolo-quinoline-dione compound that inhibits AtDLK2's enzymatic activity.

PubMed: 36172078
DOI: 10.1002/pld3.446

Experimental method

X-RAY DIFFRACTION (1.48 Å)