7TS0

Cryo-EM structure of corticotropin releasing factor receptor 2 bound to Urocortin 1 and coupled with heterotrimeric Go protein

Summary for 7TS0

• Entry DOI: 10.2210/pdb7ts0/pdb
• EMDB information: 26103 26104
• Descriptor: Corticotropin-releasing factor receptor 2,Corticotropin-releasing factor receptor 2,Corticotropin-releasing factor receptor 2,Corticotropin-releasing factor receptor 2,Human corticotropin releasing factor receptor 2, Urocortin, Dominant negative Go alpha subunit, ... (6 entities in total)
• Functional Keywords: gpcr, corticotropin releasing factor receptor 2, urocortin 1, go protein, signaling protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 6
• Total formula weight: 186297.20

Authors

Zhao, L.-H.,Lin, J.,Mao, C.,Zhou, X.E.,Ji, S.,Shen, D.,Xiao, P.,Melcher, K.,Zhang, Y.,Yu, X.,Xu, H.E. (deposition date: 2022-01-31, release date: 2022-11-09, Last modification date: 2025-05-14)

Primary citation

Zhao, L.H.,Lin, J.,Ji, S.Y.,Zhou, X.E.,Mao, C.,Shen, D.D.,He, X.,Xiao, P.,Sun, J.,Melcher, K.,Zhang, Y.,Yu, X.,Xu, H.E.
Structure insights into selective coupling of G protein subtypes by a class B G protein-coupled receptor.
Nat Commun, 13:6670-6670, 2022

PubMed Abstract: The ability to couple with multiple G protein subtypes, such as G, G, or G, by a given G protein-coupled receptor (GPCR) is critical for many physiological processes. Over the past few years, the cryo-EM structures for all 15 members of the medically important class B GPCRs, all in complex with G protein, have been determined. However, no structure of class B GPCRs with G has been solved to date, limiting our understanding of the precise mechanisms of G protein coupling selectivity. Here we report the structures of corticotropin releasing factor receptor 2 (CRF2R) bound to Urocortin 1 (UCN1), coupled with different classes of heterotrimeric G proteins, G and G. We compare these structures with the structure of CRF2R in complex with G to uncover the structural differences that determine the selective coupling of G protein subtypes by CRF2R. These results provide important insights into the structural basis for the ability of CRF2R to couple with multiple G protein subtypes.

PubMed: 36335102
DOI: 10.1038/s41467-022-33851-3

Experimental method

ELECTRON MICROSCOPY (2.8 Å)