7TQT

Coxsackievirus A21 capsid subdomain in complex with mouse polyclonal antibody pAbC-5

Summary for 7TQT

• Entry DOI: 10.2210/pdb7tqt/pdb
• EMDB information: 26065 26068 26069 26070 26071 26072
• Descriptor: pAbC-5 light chain, pAbC-5 heavy chain, VP1, ... (7 entities in total)
• Functional Keywords: polyclonal antibodies, immune complex, cryoem, virus-immune system complex, virus/immune system
• Biological source: Mus musculus; More
• Total number of polymer chains: 22
• Total formula weight: 504667.47

Authors

Antanasijevic, A.,Ward, A.B. (deposition date: 2022-01-27, release date: 2023-01-04, Last modification date: 2024-06-12)

Primary citation

Antanasijevic, A.,Schulze, A.J.,Reddy, V.S.,Ward, A.B.
High-resolution structural analysis of enterovirus-reactive polyclonal antibodies in complex with whole virions.
Pnas Nexus, 1:pgac253-pgac253, 2022

PubMed Abstract: Non-polio enteroviruses (NPEVs) cause serious illnesses in young children and neonates, including aseptic meningitis, encephalitis, and inflammatory muscle disease, among others. While over 100 serotypes have been described to date, vaccine only exists for EV-A71. Efforts toward rationally designed pan-NPEV vaccines would greatly benefit from structural biology methods for rapid and comprehensive evaluation of vaccine candidates and elicited antibody responses. Toward this goal, we introduced a cryo-electron-microscopy-based approach for structural analysis of virus- or vaccine-elicited polyclonal antibodies (pAbs) in complex with whole NPEV virions. We demonstrated the feasibility using coxsackievirus A21 and reconstructed five structurally distinct pAbs bound to the virus. The pAbs targeted two immunodominant epitopes, one overlapping with the receptor binding site. These results demonstrate that our method can be applied to map broad-spectrum polyclonal immune responses against intact virions and define potentially cross-reactive epitopes.

PubMed: 36712368
DOI: 10.1093/pnasnexus/pgac253

Experimental method

ELECTRON MICROSCOPY (4.1 Å)