7TPG

Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its ligand bound state

Summary for 7TPG

• Entry DOI: 10.2210/pdb7tpg/pdb
• EMDB information: 26054
• Descriptor: Putative cell surface polysaccharide polymerase/ligase, Fab Heavy (H) Chain, Fab Light (L) Chain, ... (4 entities in total)
• Functional Keywords: lipopolysaccharide, single-particle cryo-electron microscopy, molecular dynamics simulations, structural biology, undecaprenyl pyrophosphate, waal ligase, lipid a, o-antigen, membrane proteins, transglycosylation, glycosyltransferase, membrane protein
• Biological source: Cupriavidus metallidurans; More
• Total number of polymer chains: 3
• Total formula weight: 93325.03

Authors

Ashraf, K.U.,Nygaard, R.,Vickery, O.N.,Erramilli, S.K.,Herrera, C.M.,McConville, T.H.,Petrou, V.I.,Giacometti, S.I.,Dufrisne, M.B.,Nosol, K.,Zinkle, A.P.,Graham, C.L.B.,Loukeris, M.,Kloss, B.,Skorupinska-Tudek, K.,Swiezewska, E.,Roper, D.,Clarke, O.B.,Uhlemann, A.C.,Kossiakoff, A.A.,Trent, M.S.,Stansfeld, P.J.,Mancia, F. (deposition date: 2022-01-25, release date: 2022-04-06, Last modification date: 2022-04-27)

• Primary citation: Ashraf, K.U.,Nygaard, R.,Vickery, O.N.,Erramilli, S.K.,Herrera, C.M.,McConville, T.H.,Petrou, V.I.,Giacometti, S.I.,Dufrisne, M.B.,Nosol, K.,Zinkle, A.P.,Graham, C.L.B.,Loukeris, M.,Kloss, B.,Skorupinska-Tudek, K.,Swiezewska, E.,Roper, D.I.,Clarke, O.B.,Uhlemann, A.C.,Kossiakoff, A.A.,Trent, M.S.,Stansfeld, P.J.,Mancia, F.; Structural basis of lipopolysaccharide maturation by the O-antigen ligase.; Nature, 604:371-376, 2022; PubMed: 35388216; DOI: 10.1038/s41586-022-04555-x

Experimental method

ELECTRON MICROSCOPY (3.23 Å)