7TJQ
SAN27-14 bound to a antigenic site V on prefusion-stabilized hMPV F
Summary for 7TJQ
| Entry DOI | 10.2210/pdb7tjq/pdb |
| Related | 7TL0 |
| EMDB information | 25929 25982 |
| Descriptor | SAN27-14 Fab heavy chain, SAN27-14 Fab light chain, Fusion glycoprotein F0, ... (6 entities in total) |
| Functional Keywords | neutralizing antibodies, prefusion-specific antibodies, antigenic site v, hmpv f, viral protein-immune system complex, viral protein/immune system |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 15 |
| Total formula weight | 403422.24 |
| Authors | Hsieh, C.-L.,McLellan, J.S.,Rush, S.A. (deposition date: 2022-01-16, release date: 2022-09-14, Last modification date: 2024-10-23) |
| Primary citation | Rush, S.A.,Brar, G.,Hsieh, C.L.,Chautard, E.,Rainho-Tomko, J.N.,Slade, C.D.,Bricault, C.A.,Kume, A.,Kearns, J.,Groppo, R.,Mundle, S.T.,Zhang, L.,Casimiro, D.,Fu, T.M.,DiNapoli, J.M.,McLellan, J.S. Characterization of prefusion-F-specific antibodies elicited by natural infection with human metapneumovirus. Cell Rep, 40:111399-111399, 2022 Cited by PubMed Abstract: Human metapneumovirus (hMPV) is a major cause of acute respiratory infections in infants and older adults, for which no vaccines or therapeutics are available. The viral fusion (F) glycoprotein is required for entry and is the primary target of neutralizing antibodies; however, little is known about the humoral immune response generated from natural infection. Here, using prefusion-stabilized F proteins to interrogate memory B cells from two older adults, we obtain over 700 paired non-IgM antibody sequences representing 563 clonotypes, indicative of a highly polyclonal response. Characterization of 136 monoclonal antibodies reveals broad recognition of the protein surface, with potently neutralizing antibodies targeting each antigenic site. Cryo-EM studies further reveal two non-canonical sites and the molecular basis for recognition of the apex of hMPV F by two prefusion-specific neutralizing antibodies. Collectively, these results provide insight into the humoral response to hMPV infection in older adults and will help guide vaccine development. PubMed: 36130517DOI: 10.1016/j.celrep.2022.111399 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.13 Å) |
Structure validation
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