7TAN

Structure of VRK1 C-terminal tail bound to nucleosome core particle

7TAN の概要

• エントリーDOI: 10.2210/pdb7tan/pdb
• EMDBエントリー: 25777
• 分子名称: Histone H3.2, Histone H4, Histone H2A type 1, ... (7 entities in total)
• 機能のキーワード: nucleosome, chromatin, nuclear protein, structural protein-dna-transferase complex, structural protein/dna/transferase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 314585.58

構造登録者

Spangler, C.J.,Budziszewski, G.R.,McGinty, R.K. (登録日: 2021-12-21, 公開日: 2022-05-04, 最終更新日: 2024-02-28)

主引用文献

Budziszewski, G.R.,Zhao, Y.,Spangler, C.J.,Kedziora, K.M.,Williams, M.R.,Azzam, D.N.,Skrajna, A.,Koyama, Y.,Cesmat, A.P.,Simmons, H.C.,Arteaga, E.C.,Strauss, J.D.,Kireev, D.,McGinty, R.K.
Multivalent DNA and nucleosome acidic patch interactions specify VRK1 mitotic localization and activity.
Nucleic Acids Res., 50:4355-4371, 2022

PubMed Abstract: A key role of chromatin kinases is to phosphorylate histone tails during mitosis to spatiotemporally regulate cell division. Vaccinia-related kinase 1 (VRK1) is a serine-threonine kinase that phosphorylates histone H3 threonine 3 (H3T3) along with other chromatin-based targets. While structural studies have defined how several classes of histone-modifying enzymes bind to and function on nucleosomes, the mechanism of chromatin engagement by kinases is largely unclear. Here, we paired cryo-electron microscopy with biochemical and cellular assays to demonstrate that VRK1 interacts with both linker DNA and the nucleosome acidic patch to phosphorylate H3T3. Acidic patch binding by VRK1 is mediated by an arginine-rich flexible C-terminal tail. Homozygous missense and nonsense mutations of this acidic patch recognition motif in VRK1 are causative in rare adult-onset distal spinal muscular atrophy. We show that these VRK1 mutations interfere with nucleosome acidic patch binding, leading to mislocalization of VRK1 during mitosis, thus providing a potential new molecular mechanism for pathogenesis.

PubMed: 35390161
DOI: 10.1093/nar/gkac198

実験手法

ELECTRON MICROSCOPY (3 Å)