7T9R
Crystal structure of Crocodile defensin CpoBD13:phosphatidic acid complex
Summary for 7T9R
| Entry DOI | 10.2210/pdb7t9r/pdb |
| Related | 7T9Q |
| Descriptor | CpoBD13, 1,2-ETHANEDIOL, (R)-2-(FORMYLOXY)-3-(PHOSPHONOOXY)PROPYL PENTANOATE, ... (5 entities in total) |
| Functional Keywords | defensin, phosphatidic acid, complex, phospholipid, antimicrobial protein |
| Biological source | Crocodylus porosus |
| Total number of polymer chains | 4 |
| Total formula weight | 20030.44 |
| Authors | Kvansakul, M.,Williams, S.,Hulett, M.D. (deposition date: 2021-12-19, release date: 2023-01-18, Last modification date: 2023-10-25) |
| Primary citation | Williams, S.A.,Lay, F.T.,Bindra, G.K.,Banjara, S.,Poon, I.K.H.,Phan, T.K.,Kvansakul, M.,Hulett, M.D. Crocodile defensin (CpoBD13) antifungal activity via pH-dependent phospholipid targeting and membrane disruption. Nat Commun, 14:1170-1170, 2023 Cited by PubMed Abstract: Crocodilians are an order of ancient reptiles that thrive in pathogen-rich environments. The ability to inhabit these harsh environments is indicative of a resilient innate immune system. Defensins, a family of cysteine-rich cationic host defence peptides, are a major component of the innate immune systems of all plant and animal species, however crocodilian defensins are poorly characterised. We now show that the saltwater crocodile defensin CpoBD13 harbors potent antifungal activity that is mediated by a pH-dependent membrane-targeting action. CpoBD13 binds the phospholipid phosphatidic acid (PA) to form a large helical oligomeric complex, with specific histidine residues mediating PA binding. The utilisation of histidine residues for PA engagement allows CpoBD13 to exhibit differential activity at a range of environmental pH values, where CpoBD13 is optimally active in an acidic environment. PubMed: 36859344DOI: 10.1038/s41467-023-36280-y PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.45 Å) |
Structure validation
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