7SPS

Crystal structure of human glucose transporter GLUT3 bound with exofacial inhibitor SA47

Summary for 7SPS

• Entry DOI: 10.2210/pdb7sps/pdb
• Descriptor: Solute carrier family 2, facilitated glucose transporter member 3, methyl N-[(2-{4-[4-(5-fluoro-2-methoxyphenyl)piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidin-1-yl}phenyl)methyl]-beta-alaninate, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (4 entities in total)
• Functional Keywords: mfs, hexose transporter, inhibitor, transport protein-inhibitor complex, transport protein/inhibitor
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 116637.68

Authors

Wang, N.,Jiang, X.,Yan, N. (deposition date: 2021-11-03, release date: 2022-05-18, Last modification date: 2023-10-18)

Primary citation

Wang, N.,Zhang, S.,Yuan, Y.,Xu, H.,Defossa, E.,Matter, H.,Besenius, M.,Derdau, V.,Dreyer, M.,Halland, N.,He, K.H.,Petry, S.,Podeschwa, M.,Tennagels, N.,Jiang, X.,Yan, N.
Molecular basis for inhibiting human glucose transporters by exofacial inhibitors.
Nat Commun, 13:2632-2632, 2022

PubMed Abstract: Human glucose transporters (GLUTs) are responsible for cellular uptake of hexoses. Elevated expression of GLUTs, particularly GLUT1 and GLUT3, is required to fuel the hyperproliferation of cancer cells, making GLUT inhibitors potential anticancer therapeutics. Meanwhile, GLUT inhibitor-conjugated insulin is being explored to mitigate the hypoglycemia side effect of insulin therapy in type 1 diabetes. Reasoning that exofacial inhibitors of GLUT1/3 may be favored for therapeutic applications, we report here the engineering of a GLUT3 variant, designated GLUT3exo, that can be probed for screening and validating exofacial inhibitors. We identify an exofacial GLUT3 inhibitor SA47 and elucidate its mode of action by a 2.3 Å resolution crystal structure of SA47-bound GLUT3. Our studies serve as a framework for the discovery of GLUTs exofacial inhibitors for therapeutic development.

PubMed: 35552392
DOI: 10.1038/s41467-022-30326-3

Experimental method

X-RAY DIFFRACTION (2.3 Å)