7SPM

Replication Initiator Protein REPE54 and cognate DNA sequence with terminal three prime phosphates chemically crosslinked (30 mg/mL EDC, 12 hours, 2 doses).

Summary for 7SPM

• Entry DOI: 10.2210/pdb7spm/pdb
• Related: 7rva 7sdp 7sgc 7soz
• Descriptor: DNA (5'-D(*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*A)-3'), DNA (5'-D(*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*A)-3'), RepB family plasmid replication initiator protein, ... (5 entities in total)
• Functional Keywords: replication initiator repe complex co-crystal, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Escherichia coli; More
• Total number of polymer chains: 3
• Total formula weight: 44286.12

Authors

Ward, A.R.,Snow, C.D. (deposition date: 2021-11-02, release date: 2021-11-10, Last modification date: 2023-11-01)

• Primary citation: Orun, A.R.,Dmytriw, S.,Vajapayajula, A.,Snow, C.D.; Stabilizing DNA-Protein Co-Crystals via Intra-Crystal Chemical Ligation of the DNA; Crystals, 12:-, 2022; DOI: 10.3390/cryst12010049

Experimental method

X-RAY DIFFRACTION (3.28 Å)