7SPM

Replication Initiator Protein REPE54 and cognate DNA sequence with terminal three prime phosphates chemically crosslinked (30 mg/mL EDC, 12 hours, 2 doses).

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (5'-D(*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*A)-3')	polymer	22	6696.3	1		Escherichia coli
2	B (B)	DNA (5'-D(*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*A)-3')	polymer	22	6816.4	1		Escherichia coli
3	C (C)	RepB family plasmid replication initiator protein	polymer	263	30749.1	1	UniProt (Q0E856) Pfam (PF01051) Pfam (PF21205) UniProt (by SIFTS) (P03856)	Escherichia coli	RepE,RepE protein,Replication initiation protein,Replication initiation protein FIA,Replication initiation protein RepE,Replication initiation protein of repFIA,FIA,FIA replicon,Replication protein
4	D (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E, F (A, C)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

C: 1 - 251 (UniProt: Q0E856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 118	Arg 118	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	44261.8
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	44286.1

*Water molecules are not included.