7SPL

[2T3] Self-assembling 3D DNA triangle with three inter-junction base pairs containing the L1 junction and a zero-linked center strand

7SPL の概要

• エントリーDOI: 10.2210/pdb7spl/pdb
• 関連するPDBエントリー: 3GBI
• 分子名称: DNA (5'-D(P*GP*AP*GP*CP*AP*GP*CP*CP*TP*GP*TP*GP*AP*CP*AP*TP*CP*A)-3'), DNA (5'-D(*TP*CP*TP*GP*AP*TP*GP*TP*CP*C)-3'), DNA (5'-D(P*GP*TP*AP*CP*AP*GP*GP*CP*TP*GP*C)-3'), ... (5 entities in total)
• 機能のキーワード: tensegrity triangle, dna nanotechnology, nanomaterials, linking number, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 13102.64

構造登録者

Vecchioni, S.,Lu, B.,Sha, R.,Ohayon, Y.P.,Seeman, N.C. (登録日: 2021-11-02, 公開日: 2022-11-09, 最終更新日: 2023-10-25)

主引用文献

Vecchioni, S.,Lu, B.,Janowski, J.,Woloszyn, K.,Jonoska, N.,Seeman, N.C.,Mao, C.,Ohayon, Y.P.,Sha, R.
The Rule of Thirds: Controlling Junction Chirality and Polarity in 3D DNA Tiles.
Small, 19:e2206511-e2206511, 2023

PubMed Abstract: The successful self-assembly of tensegrity triangle DNA crystals heralded the ability to programmably construct macroscopic crystalline nanomaterials from rationally-designed, nanoscale components. This 3D DNA tile owes its "tensegrity" nature to its three rotationally stacked double helices locked together by the tensile winding of a center strand segmented into 7 base pair (bp) inter-junction regions, corresponding to two-thirds of a helical turn of DNA. All reported tensegrity triangles to date have employed turn inter-junction segments, yielding right-handed, antiparallel, "J1" junctions. Here a minimal DNA triangle motif consisting of 3-bp inter-junction segments, or one-third of a helical turn is reported. It is found that the minimal motif exhibits a reversed morphology with a left-handed tertiary structure mediated by a locally-parallel Holliday junction-the "L1" junction. This parallel junction yields a predicted helical groove matching pattern that breaks the pseudosymmetry between tile faces, and the junction morphology further suggests a folding mechanism. A Rule of Thirds by which supramolecular chirality can be programmed through inter-junction DNA segment length is identified. These results underscore the role that global topological forces play in determining local DNA architecture and ultimately point to an under-explored class of self-assembling, chiral nanomaterials for topological processes in biological systems.

PubMed: 36585389
DOI: 10.1002/smll.202206511

実験手法

X-RAY DIFFRACTION (6.09 Å)