7SGT
Domain III (EDIII) of the POWV E glycoprotein
Summary for 7SGT
| Entry DOI | 10.2210/pdb7sgt/pdb |
| Related | 7KYL |
| NMR Information | BMRB: 30959 |
| Descriptor | Envelope protein E (1 entity in total) |
| Functional Keywords | powassan virus, nanoparticle immunogen, flavivirus, monoclonal antibody, viral protein |
| Biological source | Tick-borne powassan virus (strain lb) |
| Total number of polymer chains | 1 |
| Total formula weight | 11182.76 |
| Authors | Harris, R.,Cahill, S.M.,Cowburn, D. (deposition date: 2021-10-07, release date: 2022-05-18, Last modification date: 2024-10-16) |
| Primary citation | Malonis, R.J.,Georgiev, G.I.,Haslwanter, D.,VanBlargan, L.A.,Fallon, G.,Vergnolle, O.,Cahill, S.M.,Harris, R.,Cowburn, D.,Chandran, K.,Diamond, M.S.,Lai, J.R. A Powassan virus domain III nanoparticle immunogen elicits neutralizing and protective antibodies in mice. Plos Pathog., 18:e1010573-e1010573, 2022 Cited by PubMed Abstract: Powassan virus (POWV) is an emerging tick borne flavivirus (TBFV) that causes severe neuroinvasive disease. Currently, there are no approved treatments or vaccines to combat POWV infection. Here, we generated and characterized a nanoparticle immunogen displaying domain III (EDIII) of the POWV E glycoprotein. Immunization with POWV EDIII presented on nanoparticles resulted in significantly higher serum neutralizing titers against POWV than immunization with monomeric POWV EDIII. Furthermore, passive transfer of EDIII-reactive sera protected against POWV challenge in vivo. We isolated and characterized a panel of EDIII-specific monoclonal antibodies (mAbs) and identified several that potently inhibit POWV infection and engage distinct epitopes within the lateral ridge and C-C' loop of the EDIII. By creating a subunit-based nanoparticle immunogen with vaccine potential that elicits antibodies with protective activity against POWV infection, our findings enhance our understanding of the molecular determinants of antibody-mediated neutralization of TBFVs. PubMed: 35679349DOI: 10.1371/journal.ppat.1010573 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
