7SD5

Crystallographic structure of neutralizing antibody 10-40 in complex with SARS-CoV-2 spike receptor binding domain

7SD5 の概要

• エントリーDOI: 10.2210/pdb7sd5/pdb
• 分子名称: Spike protein S1, 10-40 Heavy chain, 10-40 Light chain, ... (5 entities in total)
• 機能のキーワード: covid-19, sars-cov-2, viral protein, spike glycoprotein, receptor binding protein, rbd, neutralizing antibody, 10-40, fab, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 75782.37

構造登録者

Reddem, E.R.,Casner, R.G.,Shapiro, L. (登録日: 2021-09-29, 公開日: 2022-04-27, 最終更新日: 2024-10-23)

主引用文献

Liu, L.,Iketani, S.,Guo, Y.,Reddem, E.R.,Casner, R.G.,Nair, M.S.,Yu, J.,Chan, J.F.,Wang, M.,Cerutti, G.,Li, Z.,Morano, N.C.,Castagna, C.D.,Corredor, L.,Chu, H.,Yuan, S.,Poon, V.K.,Chan, C.C.,Chen, Z.,Luo, Y.,Cunningham, M.,Chavez, A.,Yin, M.T.,Perlin, D.S.,Tsuji, M.,Yuen, K.Y.,Kwong, P.D.,Sheng, Z.,Huang, Y.,Shapiro, L.,Ho, D.D.
An antibody class with a common CDRH3 motif broadly neutralizes sarbecoviruses.
Sci Transl Med, 14:eabn6859-eabn6859, 2022

PubMed Abstract: The devastation caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made clear the importance of pandemic preparedness. To address future zoonotic outbreaks due to related viruses in the sarbecovirus subgenus, we identified a human monoclonal antibody, 10-40, that neutralized or bound all sarbecoviruses tested in vitro and protected against SARS-CoV-2 and SARS-CoV in vivo. Comparative studies with other receptor-binding domain (RBD)-directed antibodies showed 10-40 to have the greatest breadth against sarbecoviruses, suggesting that 10-40 is a promising agent for pandemic preparedness. Moreover, structural analyses on 10-40 and similar antibodies not only defined an epitope cluster in the inner face of the RBD that is well conserved among sarbecoviruses but also uncovered a distinct antibody class with a common CDRH3 motif. Our analyses also suggested that elicitation of this class of antibodies may not be overly difficult, an observation that bodes well for the development of a pan-sarbecovirus vaccine.

PubMed: 35438546
DOI: 10.1126/scitranslmed.abn6859

実験手法

X-RAY DIFFRACTION (1.53 Å)