7S9V

DrmAB:ADP

This is a non-PDB format compatible entry.

Summary for 7S9V

• Entry DOI: 10.2210/pdb7s9v/pdb
• EMDB information: 24938
• Descriptor: DrmA, DrmB, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• Functional Keywords: disarm, helicase, immune system
• Biological source: Serratia; More
• Total number of polymer chains: 2
• Total formula weight: 217075.01

Authors

Bravo, J.P.K.,Taylor, D.W.,Brouns, S.J.J.,Aparicio-Maldonado, C. (deposition date: 2021-09-21, release date: 2022-06-15, Last modification date: 2024-06-05)

Primary citation

Bravo, J.P.K.,Aparicio-Maldonado, C.,Nobrega, F.L.,Brouns, S.J.J.,Taylor, D.W.
Structural basis for broad anti-phage immunity by DISARM.
Nat Commun, 13:2987-2987, 2022

PubMed Abstract: In the evolutionary arms race against phage, bacteria have assembled a diverse arsenal of antiviral immune strategies. While the recently discovered DISARM (Defense Island System Associated with Restriction-Modification) systems can provide protection against a wide range of phage, the molecular mechanisms that underpin broad antiviral targeting but avoiding autoimmunity remain enigmatic. Here, we report cryo-EM structures of the core DISARM complex, DrmAB, both alone and in complex with an unmethylated phage DNA mimetic. These structures reveal that DrmAB core complex is autoinhibited by a trigger loop (TL) within DrmA and binding to DNA substrates containing a 5' overhang dislodges the TL, initiating a long-range structural rearrangement for DrmAB activation. Together with structure-guided in vivo studies, our work provides insights into the mechanism of phage DNA recognition and specific activation of this widespread antiviral defense system.

PubMed: 35624106
DOI: 10.1038/s41467-022-30673-1

Experimental method

ELECTRON MICROSCOPY (3.3 Å)