7S3V

Structure of HsKYNase_66, an evolved variant of human kynureninase with greatly increased activity towards kynurenine

Summary for 7S3V

• Entry DOI: 10.2210/pdb7s3v/pdb
• Descriptor: Kynureninase (1 entity in total)
• Functional Keywords: kynurenine, tryptophan, cancer, directed-evolution, antitumor protein, hydrolase
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 106019.68

Authors

Burkholder, N.T.,Zhang, Y.J. (deposition date: 2021-09-08, release date: 2022-12-21, Last modification date: 2023-11-15)

• Primary citation: Blazeck, J.,Karamitros, C.S.,Ford, K.,Somody, C.,Qerqez, A.,Murray, K.,Burkholder, N.T.,Marshall, N.,Sivakumar, A.,Lu, W.C.,Tan, B.,Lamb, C.,Tanno, Y.,Siddiqui, M.Y.,Ashoura, N.,Coma, S.,Zhang, X.M.,McGovern, K.,Kumada, Y.,Zhang, Y.J.,Manfredi, M.,Johnson, K.A.,D'Arcy, S.,Stone, E.,Georgiou, G.; Bypassing evolutionary dead ends and switching the rate-limiting step of a human immunotherapeutic enzyme.; Nat Catal, 5:952-967, 2022; PubMed: 36465553; DOI: 10.1038/s41929-022-00856-6

Experimental method

X-RAY DIFFRACTION (3.249 Å)