7S3V

Structure of HsKYNase_66, an evolved variant of human kynureninase with greatly increased activity towards kynurenine

7S3V の概要

• エントリーDOI: 10.2210/pdb7s3v/pdb
• 分子名称: Kynureninase (1 entity in total)
• 機能のキーワード: kynurenine, tryptophan, cancer, directed-evolution, antitumor protein, hydrolase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 106019.68

構造登録者

Burkholder, N.T.,Zhang, Y.J. (登録日: 2021-09-08, 公開日: 2022-12-21, 最終更新日: 2023-11-15)

• 主引用文献: Blazeck, J.,Karamitros, C.S.,Ford, K.,Somody, C.,Qerqez, A.,Murray, K.,Burkholder, N.T.,Marshall, N.,Sivakumar, A.,Lu, W.C.,Tan, B.,Lamb, C.,Tanno, Y.,Siddiqui, M.Y.,Ashoura, N.,Coma, S.,Zhang, X.M.,McGovern, K.,Kumada, Y.,Zhang, Y.J.,Manfredi, M.,Johnson, K.A.,D'Arcy, S.,Stone, E.,Georgiou, G.; Bypassing evolutionary dead ends and switching the rate-limiting step of a human immunotherapeutic enzyme.; Nat Catal, 5:952-967, 2022; PubMed: 36465553; DOI: 10.1038/s41929-022-00856-6

実験手法

X-RAY DIFFRACTION (3.249 Å)