7RWV

Crystal structure of a metal-free RIDC1 variant

Summary for 7RWV

Entry DOI	10.2210/pdb7rwv/pdb
Descriptor	Soluble cytochrome b562, HEME C (3 entities in total)
Functional Keywords	metal binding protein
Biological source	Escherichia coli
Total number of polymer chains	4
Total formula weight	49102.46
Authors	Kakkis, A.,Golub, E. (deposition date: 2021-08-20, release date: 2022-06-29, Last modification date: 2024-11-13)
Primary citation	Kakkis, A.,Golub, E.,Choi, T.S.,Tezcan, F.A. Redox- and metal-directed structural diversification in designed metalloprotein assemblies. Chem.Commun.(Camb.), 58:6958-6961, 2022 Cited by PubMed Abstract: Herein we describe a designed protein building block whose self-assembly behaviour is dually gated by the redox state of disulphide bonds and the identity of exogenous metal ions. This protein construct is shown - through extensive structural and biophysical characterization - to access five distinct oligomeric states, exemplifying how the complex interplay between hydrophobic, metal-ligand, and reversible covalent interactions could be harnessed to obtain multiple, responsive protein architectures from a single building block. PubMed: 35642584 DOI: 10.1039/d2cc02440c PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.2 Å)

Structure validation