7RSA

STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS

7RSA の概要

• エントリーDOI: 10.2210/pdb7rsa/pdb
• 分子名称: RIBONUCLEASE A, TERTIARY-BUTYL ALCOHOL (3 entities in total)
• 機能のキーワード: hydrolase (phosphoric diester)
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13782.45

構造登録者

Wlodawer, A.,Gilliland, G.L. (登録日: 1988-06-10, 公開日: 1988-10-09, 最終更新日: 2024-05-22)

主引用文献

Wlodawer, A.,Svensson, L.A.,Sjolin, L.,Gilliland, G.L.
Structure of phosphate-free ribonuclease A refined at 1.26 A.
Biochemistry, 27:2705-2717, 1988

PubMed Abstract: The structure of phosphate-free bovine ribonuclease A has been refined at 1.26-A resolution by a restrained least-squares procedure to a final R factor of 0.15. X-ray diffraction data were collected with an electronic position-sensitive detector. The final model consists of all atoms in the polypeptide chain including hydrogens, 188 water sites with full or partial occupancy, and a single molecule of 2-methyl-2-propanol. Thirteen side chains were modeled with two alternate conformations. Major changes to the active site include the addition of two waters in the phosphate-binding pocket, disordering of Gln-11, and tilting of the imidazole ring of His-119. The structure of the protein and of the associated solvent was extensively compared with three other high-resolution, refined structures of this enzyme.

PubMed: 3401445
DOI: 10.1021/bi00408a010

実験手法

X-RAY DIFFRACTION (1.26 Å)