7RQD

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MTI-tripeptidyl-tRNA analog ACCA-ITM, and chloramphenicol at 2.50A resolution

This is a non-PDB format compatible entry.

Summary for 7RQD

• Entry DOI: 10.2210/pdb7rqd/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (63 entities in total)
• Functional Keywords: chloramphenicol; linezolid; antibiotic; peptidyl-trna; 70s ribosome; x-ray structure; inhibition of translation; uncompetitive inhibition; peptidyl transferase center; context-specificity of drug action, ribosome
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 112
• Total formula weight: 4432412.80

Authors

Syroegin, E.A.,Flemmich, L.,Klepacki, D.,Vazquez-Laslop, N.,Micura, R.,Polikanov, Y.S. (deposition date: 2021-08-06, release date: 2022-01-26, Last modification date: 2024-03-20)

• Primary citation: Syroegin, E.A.,Flemmich, L.,Klepacki, D.,Vazquez-Laslop, N.,Micura, R.,Polikanov, Y.S.; Structural basis for the context-specific action of the classic peptidyl transferase inhibitor chloramphenicol.; Nat.Struct.Mol.Biol., 29:152-161, 2022; PubMed: 35165455; DOI: 10.1038/s41594-022-00720-y

Experimental method

X-RAY DIFFRACTION (2.5 Å)