7RQ9

Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.60A resolution

This is a non-PDB format compatible entry.

Summary for 7RQ9

• Entry DOI: 10.2210/pdb7rq9/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (62 entities in total)
• Functional Keywords: iboxamycin, clindamycin, lincosamides, structure-based drug design, antibiotic, resistance, methylation, a2058, 23s rrna, 70s ribosome, inhibition of translation, peptidyl transferase center, nascent peptide exit tunnel, ribosome, ribosome-rna complex, ribosome/rna
• Biological source: Escherichia coli; More
• Total number of polymer chains: 112
• Total formula weight: 4570834.74

Authors

Mitcheltree, M.J.,Pisipati, A.,Syroegin, E.A.,Silvestre, K.J.,Klepacki, D.,Mason, J.D.,Terwilliger, D.W.,Testolin, G.,Pote, A.R.,Wu, K.J.Y.,Ladley, R.P.,Chatman, K.,Mankin, A.S.,Polikanov, Y.S.,Myers, A.G. (deposition date: 2021-08-06, release date: 2021-10-13, Last modification date: 2023-11-15)

• Primary citation: Mitcheltree, M.J.,Pisipati, A.,Syroegin, E.A.,Silvestre, K.J.,Klepacki, D.,Mason, J.D.,Terwilliger, D.W.,Testolin, G.,Pote, A.R.,Wu, K.J.Y.,Ladley, R.P.,Chatman, K.,Mankin, A.S.,Polikanov, Y.S.,Myers, A.G.; A synthetic antibiotic class overcoming bacterial multidrug resistance.; Nature, 599:507-512, 2021; PubMed: 34707295; DOI: 10.1038/s41586-021-04045-6

Experimental method

X-RAY DIFFRACTION (2.6 Å)