7RFD

E. coli peptidyl-prolyl cis-trans isomerase, mutant Phe4Ala Phe27CF3-Phe/Phe98CF3-Phe

Summary for 7RFD

• Entry DOI: 10.2210/pdb7rfd/pdb
• Related: 7N3J
• Descriptor: Peptidyl-prolyl cis-trans isomerase (2 entities in total)
• Functional Keywords: peptidyl-prolyl cis-trans isomerase, non-canonical amino acids, trifluoromethyl-phenylalanine, isomerase
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 19063.23

Authors

Frkic, R.L.,Otting, G.,Jackson, C.J. (deposition date: 2021-07-14, release date: 2021-09-29, Last modification date: 2024-11-06)

Primary citation

Orton, H.W.,Qianzhu, H.,Abdelkader, E.H.,Habel, E.I.,Tan, Y.J.,Frkic, R.L.,Jackson, C.J.,Huber, T.,Otting, G.
Through-Space Scalar 19 F- 19 F Couplings between Fluorinated Noncanonical Amino Acids for the Detection of Specific Contacts in Proteins.
J.Am.Chem.Soc., 143:19587-19598, 2021

PubMed Abstract: Fluorine atoms are known to display scalar F-F couplings in nuclear magnetic resonance (NMR) spectra when they are sufficiently close in space for nonbonding orbitals to overlap. We show that fluorinated noncanonical amino acids positioned in the hydrophobic core or on the surface of a protein can be linked by scalar through-space F-F () couplings even if the F spins are in the time average separated by more than the van der Waals distance. Using two different aromatic amino acids featuring CF groups, -trifluoromethyl-tyrosine and 4-trifluoromethyl-phenylalanine, we show that F-F TOCSY experiments are sufficiently sensitive to detect couplings between 2.5 and 5 Hz in the 19 kDa protein PpiB measured on a two-channel 400 MHz NMR spectrometer with a regular room temperature probe. A quantitative evolution experiment enables the measurement of coupling constants that are up to five times smaller than the F NMR line width. In addition, a new aminoacyl-tRNA synthetase was identified for genetic encoding of -(trifluoroacetyl)-l-lysine (TFA-Lys) and F-F TOCSY peaks were observed between two TFA-Lys residues incorporated into the proteins AncCDT-1 and mRFP despite high solvent exposure and flexibility of the TFA-Lys side chains. With the ready availability of systems for site-specific incorporation of fluorinated amino acids into proteins by genetic encoding, F-F interactions offer a straightforward way to probe the spatial proximity of selected sites without any assignments of H NMR resonances.

PubMed: 34780162
DOI: 10.1021/jacs.1c10104

Experimental method

X-RAY DIFFRACTION (1.35 Å)