7RB8
cryo-EM structure of the ADP state wild type myosin-15-F-actin complex
Summary for 7RB8
| Entry DOI | 10.2210/pdb7rb8/pdb |
| Related | 7R8V 7R91 |
| EMDB information | 24321 24322 24399 |
| Descriptor | Actin, alpha skeletal muscle, intermediate form, Isoform 3 of Unconventional myosin-XV, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total) |
| Functional Keywords | myosin motor proteins, actin cytoskeleton, stereocilia, deafness, motor protein, motor protein-atp binding protein complex, motor protein/atp binding protein |
| Biological source | Mus musculus (Mouse) More |
| Total number of polymer chains | 4 |
| Total formula weight | 204883.02 |
| Authors | Gong, R.,Reynolds, M.J.,Gurel, P.,Alushin, G.M. (deposition date: 2021-07-05, release date: 2021-07-28, Last modification date: 2022-08-03) |
| Primary citation | Gong, R.,Jiang, F.,Moreland, Z.G.,Reynolds, M.J.,de Los Reyes, S.E.,Gurel, P.,Shams, A.,Heidings, J.B.,Bowl, M.R.,Bird, J.E.,Alushin, G.M. Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia. Sci Adv, 8:eabl4733-eabl4733, 2022 Cited by PubMed Abstract: The motor protein myosin-15 is necessary for the development and maintenance of mechanosensory stereocilia, and mutations in myosin-15 cause hereditary deafness. In addition to transporting actin regulatory machinery to stereocilia tips, myosin-15 directly nucleates actin filament ("F-actin") assembly, which is disrupted by a progressive hearing loss mutation (p.D1647G, ""). Here, we present cryo-electron microscopy structures of myosin-15 bound to F-actin, providing a framework for interpreting the impacts of deafness mutations on motor activity and actin nucleation. Rigor myosin-15 evokes conformational changes in F-actin yet maintains flexibility in actin's D-loop, which mediates inter-subunit contacts, while the mutant locks the D-loop in a single conformation. Adenosine diphosphate-bound myosin-15 also locks the D-loop, which correspondingly blunts actin-polymerization stimulation. We propose myosin-15 enhances polymerization by bridging actin protomers, regulating nucleation efficiency by modulating actin's structural plasticity in a myosin nucleotide state-dependent manner. This tunable regulation of actin polymerization could be harnessed to precisely control stereocilium height. PubMed: 35857845DOI: 10.1126/sciadv.abl4733 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.63 Å) |
Structure validation
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