7R98

Structure of the SARS-CoV-2 N protein RNA-binding domain bound to single-domain antibody B6

「7N0S」から置き換えられました

7R98 の概要

• エントリーDOI: 10.2210/pdb7r98/pdb
• 分子名称: Nucleoprotein, Nanobody B6 (2 entities in total)
• 機能のキーワード: sars-cov-2, nanobody, nucleocapsid, virus, viral protein-immune system complex, rna binding protein, viral protein/immune system
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 86928.52

構造登録者

Corbett, K.D.,Ye, Q. (登録日: 2021-06-28, 公開日: 2021-07-07, 最終更新日: 2024-10-09)

主引用文献

Ye, Q.,Lu, S.,Corbett, K.D.
Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
Front Immunol, 12:719037-719037, 2021

PubMed Abstract: The COVID-19 pandemic, caused by the coronavirus SARS-CoV-2, is the most severe public health event of the twenty-first century. While effective vaccines against SARS-CoV-2 have been developed, there remains an urgent need for diagnostics to quickly and accurately detect infections. Antigen tests, particularly those that detect the abundant SARS-CoV-2 Nucleocapsid protein, are a proven method for detecting active SARS-CoV-2 infections. Here we report high-resolution crystal structures of three llama-derived single-domain antibodies that bind the SARS-CoV-2 Nucleocapsid protein with high affinity. Each antibody recognizes a specific folded domain of the protein, with two antibodies recognizing the N-terminal RNA binding domain and one recognizing the C-terminal dimerization domain. The two antibodies that recognize the RNA binding domain affect both RNA binding affinity and RNA-mediated phase separation of the Nucleocapsid protein. All three antibodies recognize highly conserved surfaces on the Nucleocapsid protein, suggesting that they could be used to develop affordable diagnostic tests to detect all circulating SARS-CoV-2 variants.

PubMed: 34381460
DOI: 10.3389/fimmu.2021.719037

実験手法

X-RAY DIFFRACTION (2.51 Å)