7R53

Crystal structure of human TLR8 in complex with Compound 15

Summary for 7R53

• Entry DOI: 10.2210/pdb7r53/pdb
• Descriptor: Toll-like receptor 8, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: toll-like receptor, tlr8, immune system
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 192845.35

Authors

Faller, M.,Zink, F. (deposition date: 2022-02-10, release date: 2022-03-23, Last modification date: 2024-11-13)

Primary citation

Betschart, C.,Faller, M.,Zink, F.,Hemmig, R.,Blank, J.,Vangrevelinghe, E.,Bourrel, M.,Glatthar, R.,Behnke, D.,Barker, K.,Heizmann, A.,Angst, D.,Nimsgern, P.,Jacquier, S.,Junt, T.,Zipfel, G.,Ruzzante, G.,Loetscher, P.,Limonta, S.,Hawtin, S.,Andre, C.B.,Boulay, T.,Feifel, R.,Knoepfel, T.
Structure-Based Optimization of a Fragment-like TLR8 Binding Screening Hit to an In Vivo Efficacious TLR7/8 Antagonist.
Acs Med.Chem.Lett., 13:658-664, 2022

PubMed Abstract: Inappropriate activation of TLR7 and TLR8 is linked to several autoimmune diseases, such as lupus erythematosus. Here we report on the efficient structure-based optimization of the inhibition of TLR8, starting from a co-crystal structure of a small screening hit. Further optimization of the physicochemical properties for cellular potency and expansion of the structure-activity relationship for dual potency finally resulted in a highly potent TLR7/8 antagonist with demonstrated efficacy after oral dosing.

PubMed: 35450354
DOI: 10.1021/acsmedchemlett.1c00696

Experimental method

X-RAY DIFFRACTION (3.121 Å)