7QXM

Crystal structure of the Vibrio cholerae replicative helicase (DnaB)

Summary for 7QXM

• Entry DOI: 10.2210/pdb7qxm/pdb
• Related: 6T66
• Descriptor: Replicative DNA helicase (1 entity in total)
• Functional Keywords: helicase, replication
• Biological source: Vibrio cholerae
• Total number of polymer chains: 6
• Total formula weight: 317625.87

Authors

Legrand, P.,Quevillon-Cheruel, S.,Walbott, H.,Cargemel, C. (deposition date: 2022-01-26, release date: 2022-05-25, Last modification date: 2024-01-31)

Primary citation

Cargemel, C.,Walbott, H.,Durand, D.,Legrand, P.,Ouldali, M.,Ferat, J.L.,Marsin, S.,Quevillon-Cheruel, S.
The apo-form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive planar trimer of dimers.
Febs Lett., 596:2031-2040, 2022

PubMed Abstract: To enable chromosomal replication, DNA is unwound by the ATPase molecular motor replicative helicase. The bacterial helicase DnaB is a ring-shaped homo-hexamer whose conformational dynamics are being studied through its different 3D structural states adopted along its functional cycle. Our findings describe a new crystal structure for the apo-DnaB from Vibrio cholerae, forming a planar hexamer with pseudo-symmetry, constituted by a trimer of dimers in which the C-terminal domains delimit a triskelion-shaped hole. This hexamer is labile and inactive. We suggest that it represents an intermediate state allowing the formation of the active NTP-bound hexamer from dimers.

PubMed: 35568982
DOI: 10.1002/1873-3468.14403

Experimental method

X-RAY DIFFRACTION (3.8 Å)