7QXD

Recognition of Staphylococcus aureus wall teichoic acid analogue SA475 (compound 2) by Fab4497

This is a non-PDB format compatible entry.

Summary for 7QXD

• Entry DOI: 10.2210/pdb7qxd/pdb
• Descriptor: Antibody Fab 4497 heavy chain, Antibody Fab 4497 light chain, [(2~{S},3~{S},4~{R})-4-[(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]oxy-5-[[(2~{S},3~{R})-2,3-bis(oxidanyl)butoxy]-oxidanyl-phosphoryl]oxy-2,3-bis(oxidanyl)pentyl] [(2~{R},3~{S},4~{S})-2,3,4,5-tetrakis(oxidanyl)pentyl] hydrogen phosphate, ... (7 entities in total)
• Functional Keywords: antibody, fab fragment, teichoic acid analogue, carbohydrate
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 100803.37

Authors

Soriano-Maldonado, P.,van Raaij, M.J. (deposition date: 2022-01-26, release date: 2022-08-31, Last modification date: 2024-01-31)

Primary citation

Di Carluccio, C.,Soriano-Maldonado, P.,Berni, F.,de Haas, C.J.C.,Temming, A.R.,Hendriks, A.,Ali, S.,Molinaro, A.,Silipo, A.,van Sorge, N.M.,van Raaij, M.J.,Codee, J.D.C.,Marchetti, R.
Antibody Recognition of Different Staphylococcus aureus Wall Teichoic Acid Glycoforms.
Acs Cent.Sci., 8:1383-1392, 2022

PubMed Abstract: Wall teichoic acids (WTAs) are glycopolymers decorating the surface of Gram-positive bacteria and potential targets for antibody-mediated treatments against , including methicillin-resistant (MRSA) strains. Through a combination of glycan microarray, synthetic chemistry, crystallography, NMR, and computational studies, we unraveled the molecular and structural details of fully defined synthetic WTA fragments recognized by previously described monoclonal antibodies (mAbs 4461 and 4497). Our results unveiled the structural requirements for the discriminatory recognition of α- and β-GlcNAc-modified WTA glycoforms by the complementarity-determining regions (CDRs) of the heavy and light chains of the mAbs. Both mAbs interacted not only with the sugar moiety but also with the phosphate groups as well as residues in the ribitol phosphate (RboP) units of the WTA backbone, highlighting their significant role in ligand specificity. Using elongated WTA fragments, containing two sugar modifications, we also demonstrated that the internal carbohydrate moiety of α-GlcNAc-modified WTA is preferentially accommodated in the binding pocket of mAb 4461 with respect to the terminal moiety. Our results also explained the recently documented cross-reactivity of mAb 4497 for β-1,3/β-1,4-GlcNAc-modified WTA, revealing that the flexibility of the RboP backbone is crucial to allow positioning of both glycans in the antibody binding pocket.

PubMed: 36313161
DOI: 10.1021/acscentsci.2c00125

Experimental method

X-RAY DIFFRACTION (1.649 Å)