7QPG
Human RZZ kinetochore corona complex.
Summary for 7QPG
| Entry DOI | 10.2210/pdb7qpg/pdb |
| EMDB information | 14120 |
| Descriptor | Protein zwilch homolog, Kinetochore-associated protein 1, Centromere/kinetochore protein zw10 homolog (3 entities in total) |
| Functional Keywords | kinetochore, centromere, chromosome segregation, mitosis, cell cycle |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 6 |
| Total formula weight | 872079.41 |
| Authors | Raisch, T.,Ciossani, G.,d'Amico, E.,Cmetowski, V.,Carmignani, S.,Maffini, S.,Merino, F.,Wohlgemuth, S.,Vetter, I.R.,Raunser, S.,Musacchio, A. (deposition date: 2022-01-04, release date: 2022-03-16, Last modification date: 2024-07-17) |
| Primary citation | Raisch, T.,Ciossani, G.,d'Amico, E.,Cmentowski, V.,Carmignani, S.,Maffini, S.,Merino, F.,Wohlgemuth, S.,Vetter, I.R.,Raunser, S.,Musacchio, A. Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores. Embo J., 41:e110411-e110411, 2022 Cited by PubMed Abstract: In metazoans, a ≈1 megadalton (MDa) multiprotein complex comprising the dynein-dynactin adaptor Spindly and the ROD-Zwilch-ZW10 (RZZ) complex is the building block of a fibrous biopolymer, the kinetochore fibrous corona. The corona assembles on mitotic kinetochores to promote microtubule capture and spindle assembly checkpoint (SAC) signaling. We report here a high-resolution cryo-EM structure that captures the essential features of the RZZ complex, including a farnesyl-binding site required for Spindly binding. Using a highly predictive in vitro assay, we demonstrate that the SAC kinase MPS1 is necessary and sufficient for corona assembly at supercritical concentrations of the RZZ-Spindly (RZZS) complex, and describe the molecular mechanism of phosphorylation-dependent filament nucleation. We identify several structural requirements for RZZS polymerization in rings and sheets. Finally, we identify determinants of kinetochore localization and corona assembly of Spindly. Our results describe a framework for the long-sought-for molecular basis of corona assembly on metazoan kinetochores. PubMed: 35373361DOI: 10.15252/embj.2021110411 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
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