7QHM

Cytochrome bcc-aa3 supercomplex (respiratory supercomplex III2/IV2) from Corynebacterium glutamicum (stigmatellin and azide bound)

7QHM の概要

• エントリーDOI: 10.2210/pdb7qhm/pdb
• EMDBエントリー: 13976
• 分子名称: Cytochrome bc1 complex Rieske iron-sulfur subunit, Hypothetical membrane protein, Actinobacterial supercomplex, subunit C (AscC), ... (35 entities in total)
• 機能のキーワード: supercomplex, respiratory chain, cytochrome bcc-aa3 supercomplex, proton translocation, bioenergetics, membrane protein, electron transport, oxidoreductase
• 由来する生物種: Corynebacterium glutamicum ATCC 13032; 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 774241.38

構造登録者

Kao, W.-C.,Hunte, C. (登録日: 2021-12-13, 公開日: 2022-05-18, 最終更新日: 2024-10-09)

主引用文献

Kao, W.C.,Ortmann de Percin Northumberland, C.,Cheng, T.C.,Ortiz, J.,Durand, A.,von Loeffelholz, O.,Schilling, O.,Biniossek, M.L.,Klaholz, B.P.,Hunte, C.
Structural basis for safe and efficient energy conversion in a respiratory supercomplex.
Nat Commun, 13:545-545, 2022

PubMed Abstract: Proton-translocating respiratory complexes assemble into supercomplexes that are proposed to increase the efficiency of energy conversion and limit the production of harmful reactive oxygen species during aerobic cellular respiration. Cytochrome bc complexes and cytochrome aa oxidases are major drivers of the proton motive force that fuels ATP generation via respiration, but how wasteful electron- and proton transfer is controlled to enhance safety and efficiency in the context of supercomplexes is not known. Here, we address this question with the 2.8 Å resolution cryo-EM structure of the cytochrome bcc-aa (III-IV) supercomplex from the actinobacterium Corynebacterium glutamicum. Menaquinone, substrate mimics, lycopene, an unexpected Q site, dioxygen, proton transfer routes, and conformational states of key protonable residues are resolved. Our results show how safe and efficient energy conversion is achieved in a respiratory supercomplex through controlled electron and proton transfer. The structure may guide the rational design of drugs against actinobacteria that cause diphtheria and tuberculosis.

PubMed: 35087070
DOI: 10.1038/s41467-022-28179-x

実験手法

ELECTRON MICROSCOPY (2.8 Å)