7QGF

Cubic Insulin SAD phasing at 14.2 keV

This is a non-PDB format compatible entry.

Summary for 7QGF

• Entry DOI: 10.2210/pdb7qgf/pdb
• Descriptor: Insulin A chain, Insulin B chain (3 entities in total)
• Functional Keywords: anomalous phasing sulfur-sad sulphur-sad sad helical collection, hormone
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 5716.55

Authors

Nanao, M.H.,Basu, S. (deposition date: 2021-12-08, release date: 2022-06-01, Last modification date: 2024-11-13)

Primary citation

Nanao, M.,Basu, S.,Zander, U.,Giraud, T.,Surr, J.,Guijarro, M.,Lentini, M.,Felisaz, F.,Sinoir, J.,Morawe, C.,Vivo, A.,Beteva, A.,Oscarsson, M.,Caserotto, H.,Dobias, F.,Flot, D.,Nurizzo, D.,Gigmes, J.,Foos, N.,Siebrecht, R.,Roth, T.,Theveneau, P.,Svensson, O.,Papp, G.,Lavault, B.,Cipriani, F.,Barrett, R.,Clavel, C.,Leonard, G.
ID23-2: an automated and high-performance microfocus beamline for macromolecular crystallography at the ESRF.
J.Synchrotron Radiat., 29:581-590, 2022

PubMed Abstract: ID23-2 is a fixed-energy (14.2 keV) microfocus beamline at the European Synchrotron Radiation Facility (ESRF) dedicated to macromolecular crystallography. The optics and sample environment have recently been redesigned and rebuilt to take full advantage of the upgrade of the ESRF to the fourth generation Extremely Brilliant Source (ESRF-EBS). The upgraded beamline now makes use of two sets of compound refractive lenses and multilayer mirrors to obtain a highly intense (>10 photons s) focused microbeam (minimum size 1.5 µm × 3 µm full width at half-maximum). The sample environment now includes a FLEX-HCD sample changer/storage system, as well as a state-of-the-art MD3Up high-precision multi-axis diffractometer. Automatic data reduction and analysis are also provided for more advanced protocols such as synchrotron serial crystallographic experiments.

PubMed: 35254323
DOI: 10.1107/S1600577522000984

Experimental method

X-RAY DIFFRACTION (1.203 Å)