7QG6

Co-crystal structure of UPF3A-RRM-NOPS-L with UPF2-MIF4GIII

7QG6 の概要

• エントリーDOI: 10.2210/pdb7qg6/pdb
• 分子名称: Regulator of nonsense transcripts 3A, Regulator of nonsense transcripts 2, TRIETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: nonsense mediated mrna decay neurological development x-linked intellectual disability up-frameshift proteins, rna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 234971.47

構造登録者

Powers, K.T.,Bufton, J.C.,Szeto, J.A.,Schaffitzel, C. (登録日: 2021-12-07, 公開日: 2022-07-20, 最終更新日: 2024-01-31)

主引用文献

Bufton, J.C.,Powers, K.T.,Szeto, J.A.,Toelzer, C.,Berger, I.,Schaffitzel, C.
Structures of nonsense-mediated mRNA decay factors UPF3B and UPF3A in complex with UPF2 reveal molecular basis for competitive binding and for neurodevelopmental disorder-causing mutation.
Nucleic Acids Res., 50:5934-5947, 2022

PubMed Abstract: UPF3 is a key nonsense-mediated mRNA decay (NMD) factor required for mRNA surveillance and eukaryotic gene expression regulation. UPF3 exists as two paralogs (A and B) which are differentially expressed depending on cell type and developmental stage and believed to regulate NMD activity based on cellular requirements. UPF3B mutations cause intellectual disability. The underlying molecular mechanisms remain elusive, as many of the mutations lie in the poorly characterized middle-domain of UPF3B. Here, we show that UPF3A and UPF3B share structural and functional homology to paraspeckle proteins comprising an RNA-recognition motif-like domain (RRM-L), a NONA/paraspeckle-like domain (NOPS-L), and extended α-helical domain. These domains are essential for RNA/ribosome-binding, RNA-induced oligomerization and UPF2 interaction. Structures of UPF2's third middle-domain of eukaryotic initiation factor 4G (MIF4GIII) in complex with either UPF3B or UPF3A reveal unexpectedly intimate binding interfaces. UPF3B's disease-causing mutation Y160D in the NOPS-L domain displaces Y160 from a hydrophobic cleft in UPF2 reducing the binding affinity ∼40-fold compared to wildtype. UPF3A, which is upregulated in patients with the UPF3B-Y160D mutation, binds UPF2 with ∼10-fold higher affinity than UPF3B reliant mainly on NOPS-L residues. Our characterization of RNA- and UPF2-binding by UPF3's middle-domain elucidates its essential role in NMD.

PubMed: 35640974
DOI: 10.1093/nar/gkac421

実験手法

X-RAY DIFFRACTION (2.95 Å)